Protocols
Curated laboratory protocols for PCR, RT‑PCR, LAMP, NGS, Digital PCR, and CRISPR‑PCR.
PCR Setup Checklist
Template preparation, primer design, cycling parameters, and control strategies for reliable amplification.
RT‑PCR Workflow
RNA extraction, cDNA synthesis, amplification steps, and quality control for expression and viral detection.
LAMP Pathogen Detection
Isothermal amplification protocol, primer set design, and endpoint/readout methods for rapid diagnostics.
NGS Library Preparation
Fragmentation, end‑repair, adapter ligation, and cleanup workflow for sequencing-ready libraries.
Digital PCR Quantification
Partitioning strategy, Poisson statistics, and absolute quantification best practices.
CRISPR‑PCR Assay
Guide RNA design, specificity checks, and detection strategies integrating CRISPR with PCR.